News & Events
Journal Club Presentation
Speaker(s): Dr. Guanglei QIU
When: 29 August 2017 (9.00 AM)
Where: SCELSE, B3 Meeting Room - 1
Type: Environmental Engineering Group Meetings

Title: Metabolism and ecological niche of  and  Accumulibacter in enhanced biological phosphorus removal.

Publication Information: Ricardo Marques, Jorge Santos, Hien Nguyen, Gilda Carvalho, J.P. Noronha, Per Halkjær Nielsen, Maria A.M. Reis, Adrian Oehmen, Water Research, Volume 122, 2017, Pages 159-171.

Abstract

Tetrasphaera and Candidatus Accumulibacter are two abundant polyphosphate accumulating organisms in full-scale enhanced biological phosphorus removal (EBPR) systems. However, little is known about the metabolic behaviour and ecological niche that each organism exhibits in mixed communities. In this study, an enriched culture of Tetrasphaeraand Ca. Accumulibacter was obtained using casein hydrolysate as sole carbon source. This culture was able to achieve a high phosphorus removal efficiency (>99%), storing polyphosphate while consuming amino acids anaerobically. Microautoradiography and fluorescence in situ hybridisation confirmed that more than 90% Tetrasphaera cells were responsible for amino acid consumption while Ca. Accumulibacter likely survived on fermentation products. Tetrasphaera performed the majority of the P removal (approximately 80%) in this culture, and batch tests showed that the metabolism of some carbon sources could actually lead to anaerobic orthophosphate (Pi) uptake (9.0 ± 2.1 mg-P/L) through energy generated by fermentation of glucose and amino acids. This anaerobic Pi uptake may lead to lower net Pi release to C uptake ratios and reduce the Pi needed to be removed aerobically in WWTPs. Intracellular metabolites such as amino acids, sugars, volatile fatty acids and small amines were observed as potential storage products, which may serve as energy sources in the aerobic phase. Evidence of the urea cycle was found, which could be involved in reducing the intracellular nitrogen content. This study improves our understanding of how phosphorus is removed in EBPR systems and can enable novel process optimisation strategies.