General
Research

Research Assistant (Next-Gen Sequencing)

The Singapore Centre for Environmental Life Sciences Engineering (SCELSE) is a unique interdisciplinary Research Centre of Excellence (RCE), funded by National Research Foundation, Ministry of Education, Nanyang Technological University (NTU) and National University of Singapore (NUS). Hosted by NTU in partnership with NUS, SCELSE is linking new insights from the life sciences with expertise from emerging technologies in engineering and natural sciences to understand, harness and control microbial biofilm communities.

We are seeking to appoint a talented individual to join our team at SCELSE as a Research Assistant (Next-Gen Sequencing).

Key Responsibilities:

  • Quality control of incoming samples
  • DNA, RNA and amplicon library construction for various next-generation sequencing platforms
  • Operation of next-generation sequencing instruments and associated equipment
  • Troubleshooting and correction of suboptimal results under the guidance of scientific staff
  • Assistance in the improvement of existing protocols for sample preparation and library construction
  • Quantification of nucleic acids by real time PCR
  • DNA and RNA isolation from various sources
  • General laboratory and instrument maintenance

Requirements:

  • Bachelor’s degree in Biological Sciences or related field and/or combination of education and experience
  • Strong background in Genomics and Molecular Biology required
  • Solid knowledge and hands-on experience with molecular biological methods such as handling nucleic acids (DNA and RNA), enzymatic manipulation of nucleic acids, PCR and qPCR required
  • Hands-on experience with library preparation as well as the operation of next-generation sequencing platforms required
  • Hands-on experience with liquid handling platforms preferred
  • Desire and ability to learn and adapt to rapidly changing protocols and technologies
  • Ability to work independently as well as in a team setting
  • Excellent organisational, documentation and communication skills

Interested candidates should submit a current CV including potential references and submit a cover letter with the subject heading “Application for Research Assistant (Next-Gen Sequencing)” to SCELSE-HR@ntu.edu.sg.


Research Associate (Laboratory Automation)

The Singapore Centre for Environmental Life Sciences Engineering (SCELSE) is a unique interdisciplinary Research Centre of Excellence (RCE), funded by National Research Foundation, Ministry of Education, Nanyang Technological University (NTU) and National University of Singapore (NUS). Hosted by NTU in partnership with NUS, SCELSE is linking new insights from the life sciences with expertise from emerging technologies in engineering and natural sciences to understand, harness and control microbial biofilm communities.

We are seeking to appoint a talented individual to join our team at SCELSE as a Research Assistant (Laboratory Automation).

Key Responsibilities:

  • Develop new and improve existing protocols for nucleic acid extraction, NGS library preparation, PCR set-up and clean-up as well as qPCR set-up on our existing liquid handling platforms (Agilent, Tecan and Beckman Coulter)
  • Additional responsibilities include routine maintenance, calibration and verification of the automated platforms with the help of the manufacturers
  • Possibility of getting involved in manual and automated preparation of NGS libraries as well as the set-up of NGS sequencing runs on various sequencing platforms

Requirements:

  • Master’s degree in Biotechnology, Molecular Biology, Biochemistry, Engineering or closely related discipline and or combination of education and experience
  • Ability to develop application-specific protocols on various liquid handling platforms
  • Experience with operating and scripting liquid handling systems from Agilent, Tecan and/or Beckman Coulter is required
  • Experience with multiple computer programming languages preferred
  • Ability to troubleshoot and optimise performance of instrumentation
  • Fundamental knowledge of molecular biology protocols and practices, including nucleic acid extraction, purification and quantification, PCR and next-generation sequencing sample preparation workflows strongly preferred
  • Ability to work independently and prioritise tasks efficiently
  • Good general laboratory and organisational skills

Interested candidates should submit a current CV including potential references and submit a cover letter with the subject heading “Application for Research Associate (Laboratory Automation)” to SCELSE-HR@ntu.edu.sg.


Research Engineer (High-Performance Computing)

The Singapore Centre for Environmental Life Sciences Engineering (SCELSE) is a unique interdisciplinary Research Centre of Excellence (RCE), funded by National Research Foundation, Ministry of Education, Nanyang Technological University (NTU) and National University of Singapore (NUS). SCELSE work towards discovering, controlling and directing the behaviour of biofilm communities for sustainable environmental, engineering, public health and medical applications. This is enabled through its strengths in analysis of complex microbiomes, using advanced computational biology and cutting edge facilities in genome sequencing, bio-imaging and bioreactor processes.

SCELSE hosts a genome sequencing facility with multiple state-of-the-art instruments generating petabytes of data every year, and is supported by a high-performance computing (HPC) system and multi-petabytes high-performance data storage system. The HPC environment is composed of multi-vendor resources with numerous specialised hardware and software components. 

SCELSE seeks a research engineer who will be responsible for the day-to-day maintenance, configuration, planning and implementation of mission critical computer systems infrastructure to support the genome sequencing laboratory.

In their role, the research engineer will perform installation, hardware and software integration, maintenance, administration, troubleshooting and operation of both software and hardware systems, including thousand-cores computational systems, scheduling environment and low-latency high-speed networks. The research engineer will be involved in managing system architecture design and implementation projects, working closely with high performance file systems, mass storage, operations, and user services to ensure that systems remain highly available and usable.

The research engineer will also be required to develop web-based documentation of system procedures and applications, programs to support automated system maintenance, monitor system resources and usage, and implement system security policies.

Requirements:

  • Bachelor’s degree and three to five years of progressive experience or equivalent combination of education and experience in one or more of the following fields: computer science, mathematics, computer engineering, information sciences, software engineering, or equivalent
  • Experience with Linux operating system environment in a multi-vendor environment
  • Experience with job scheduling system
  • Experience with the installation and management in a Linux-based HPC environment
  • Experience administering a production cluster environment
  • Experience with high-performance parallel file system
  • Experience in common scripting and programming languages and ANSI/GNU C, Python, Perl, or others
  • Demonstrated knowledge in low-latency networking and computer security is a plus
  • Familiarity with genomics field of research is a plus
  • Strong project management skill
  • Strong analytical skills and meticulous attitude
  • Able to work independently or in a team with minimal supervision
  • Good communication, writing and presentation skills

Interested candidates should submit a current CV including potential references and a cover letter with the subject heading “Application for Research Engineer (High-Performance Computing)” to SCELSE-HR@ntu.edu.sg.


Research Fellow (Bacterial Pathogenesis)

The Singapore Centre for Environmental Life Sciences Engineering (SCELSE) is a unique interdisciplinary Research Centre of Excellence (RCE), funded by Singapore’s National Research Foundation, Ministry of Education, Nanyang Technological University (NTU) and National University of Singapore (NUS). Hosted by NTU in partnership with NUS, SCELSE is linking new insights from the life sciences with expertise from emerging technologies in engineering, natural sciences and public health to understand, harness and control microbial biofilm communities.

We are seeking an individual to join us as a research fellow to study how Enterococcus faecalis interacts with mammalian cells during chronic biofilm-associated infections. The ideal candidate should have experience studying microbial interactions with mammalian cells. This project will involve mammalian cell culture, animal models of infection, imaging, and RNA and/or transposon sequencing experiments.

Key Requirements include:

  • PhD in microbiology, immunology, or cell biology.
  • At least 3-5 years’ experience in an academic setting is preferred.
  • Experience in genetic manipulation of bacteria.
  • Experience in mammalian cell culture.
  • Experience in working with mice. 

Interested candidates should submit a current CV including potential references and must submit a cover letter with the subject heading “Application for Research Fellow (Bacterial Pathogenesis)” to SCELSE-HR@ntu.edu.sg.The cover letter should outline why the applicant is especially suited for this project and also discuss professional goals.