General
Research

Research Fellow (Microbially Influenced Corrosion)

The Singapore Centre for Environmental Life Sciences Engineering (SCELSE) is a unique interdisciplinary Research Centre of Excellence (RCE), funded by National Research Foundation, Ministry of Education, Nanyang Technological University (NTU) and National University of Singapore (NUS). Hosted by NTU in partnership with NUS, SCELSE is linking new insights from the life sciences with expertise from emerging technologies in engineering and natural sciences to understand, harness and control microbial biofilm communities.

SCELSE is looking for a highly motivated Research Fellow to work in the area of Microbially Influenced Corrosion to join an interdisciplinary team of microbiologists, materials scientists and electrochemists.  In this project, we will combine approaches from a range of disciplines to better characterise the role of microbial biofilm communities in mediating corrosion of metal surfaces, with a specific focus of conditions that mimic the deep sea e.g. high hydrostatic pressure and low nutrient conditions.

The project will involve working with a team of researchers and hence the candidate must have excellent communication skills (oral and written) and will be expected to integrate with the team to meet objectives and timelines. The candidate will be responsible for both laboratory work as well as preparing reports and data for presentation. The candidate is also expected to learn operational knowledge of electrochemistry and corrosion monitoring.

Requirements:

  • The candidate should have a PhD in Microbiology, Biotechnology or in a related field.

  • The successful candidate should have a demonstrated track record of working with microbial communities and a clear grasp of redox processes in the context of microbial metabolic processes and interactions.

  • The ability to conduct experiments using next generation sequencing technologies as well as imaging based approaches is essential e.g. FISH or other imaging approaches such as electron microscopy.

  • Experience in cultivating organisms under high hydrostatic pressure, and/or a thorough understanding of biofilms would be advantageous.

Interested candidates should submit a current CV including potential references and submit a cover letter that addresses the selection criteria with the subject heading “Application for Research Fellow (Microbially Influenced Corrosion)” to SCELSE-HR@ntu.edu.sg.


Research Fellow (Spectroscopy)

The Singapore Centre for Environmental Life Sciences Engineering (SCELSE) is a unique interdisciplinary Research Centre of Excellence (RCE), funded by National Research Foundation, Ministry of Education, Nanyang Technological University (NTU) and National University of Singapore (NUS). Hosted by NTU in partnership with NUS, SCELSE is linking new insights from the life sciences with expertise from emerging technologies in engineering and natural sciences to understand, harness and control microbial biofilm communities.

We are seeking a Research Fellow in imaging, spectroscopy and sensing.

Description:

  • In charge of designing and building a bespoke Brillouin/fluorescent confocal microscope to study development of biofilms

  • Develop software environment to drive the microscope & acquire and analyse data

  • Drive development of spectroscopic methods (Raman, FTIR, mass spectrometry, etc) to detect presence of water in biofilms and other specimens

  • Support development of chemical sensors and tags to identify presence of metabolites and other chemicals/single molecules in biofilms

  • Support development of techniques for biochemical detection and sensing

Requirements:

  • A recognised PhD degree in Chemistry/Physics/Science

  • Strong background in relevant spectroscopy, fluorescence and confocal microscopy

  • Background in single-molecule fluorescence biophysics and/or analytical chemistry

  • Working knowledge of fluorescent proteins

  • Experience in working with bacterial cultures and biofilms

  • Experience in programming MATLAB, Python, or similar high-level programming languages

  • Independent and self-motivated

  • Possess strong written and verbal communication skills with good interpersonal skills

Interested candidates should submit a cover letter describing their research experience, interests, and a curriculum vitae with the subject heading “Application for Research Fellow (Spectroscopy)” to SCELSE-HR@ntu.edu.sg.


Research Assistant (Next-Gen Sequencing) 

The Singapore Centre for Environmental Life Sciences Engineering (SCELSE) is a unique interdisciplinary Research Centre of Excellence (RCE), funded by National Research Foundation, Ministry of Education, Nanyang Technological University (NTU) and National University of Singapore (NUS). Hosted by NTU in partnership with NUS, SCELSE is linking new insights from the life sciences with expertise from emerging technologies in engineering and natural sciences to understand, harness and control microbial biofilm communities.

We are seeking to appoint a talented individual to join our team at SCELSE as a Research Assistant (Next-Gen Sequencing).

Key Responsibilities:

  • Quality control of incoming samples
  • DNA, RNA and amplicon library construction for various next-generation sequencing platforms
  • Operation of next-generation sequencing instruments and associated equipment
  • Troubleshooting and correction of suboptimal results under the guidance of scientific staff
  • Assistance in the improvement of existing protocols for sample preparation and library construction
  • Quantification of nucleic acids by real time PCR
  • DNA and RNA isolation from various sources
  • General laboratory and instrument maintenance

Requirements:

  • BSc degree in Biological Sciences or related field and/or combination of education and experience
  • Strong background in Genomics and Molecular Biology required
  • Solid knowledge and hands-on experience with molecular biological methods such as handling nucleic acids (DNA and RNA), enzymatic manipulation of nucleic acids, PCR and qPCR required
  • Hands-on experience with library preparation as well as the operation of next-generation sequencing platforms required
  • Hands-on experience with liquid handling platforms preferred
  • Desire and ability to learn and adapt to rapidly changing protocols and technologies
  • Ability to work independently as well as in a team setting
  • Excellent organisational, documentation and communication skills

Interested candidates should submit a current CV including potential references and submit a cover letter with the subject heading “Application for Research Assistant (Next-Gen Sequencing)” to SCELSE-HR@ntu.edu.sg

 


Research Assistant (High-Throughput Sequencing Lab)

The Singapore Centre for Environmental Life Sciences Engineering (SCELSE) is a unique interdisciplinary Research Centre of Excellence (RCE), funded by National Research Foundation, Ministry of Education, Nanyang Technological University (NTU) and National University of Singapore (NUS). Hosted by the NTU in partnership with NUS, SCELSE is linking new insights from the life sciences with expertise from emerging technologies in engineering and natural sciences to understand, harness and control microbial biofilm communities.

We seek to appoint a talented individual to join our team at SCELSE as a Research Assistant (High-Throughput Sequencing Laboratory).

Key Responsibilities:

  •           Quality control of incoming DNA and RNA samples

  •           Manual and automated library construction for various sequencing platforms

  •           Quality control of finished libraries by real time PCR

  •           Setting-up of sequencing runs and operation of the latest state-of-the-art sequencing instruments

  •           Tracking of samples, reagents and sequencing data through a LIMS system

  •           Troubleshooting and correction of suboptimal results under the guidance of scientific staff

  •           General laboratory and instrument maintenance

Requirements:

  •           Bachelor’s degree in Biological Sciences or related field and/or combination of education and experience

  •           Strong background in Genomics and Molecular Biology required

  •           Solid knowledge and hands-on experience with molecular biological methods such as handling nucleic acids (DNA and RNA), enzymatic manipulation of nucleic acids, PCR and qPCR required

  •           Hands-on experience with library preparation and/or operation of next-generation sequencing platforms would be an advantage

  •           Hands-on experience with liquid handling platforms would be highly valued

  •           Desire and ability to learn and adapt to rapidly changing protocols and technologies

  •           A high level of attention to detail is absolutely mandatory

  •           Ability to work with large number of samples

  •           Ability to work independently as well as in a team setting

  •           Excellent organisational, documentation and communication skills

Interested candidates should submit a current CV including potential references and must submit a cover letter with the subject heading “Application for Research Assistant (High-Throughput Sequencing Lab)” to SCELSE-HR@ntu.edu.sg

 


Research Assistant (Cell Culture and Microbiology)

The Singapore Centre for Environmental Life Sciences Engineering (SCELSE) is a unique interdisciplinary Research Centre of Excellence (RCE), funded by National Research Foundation, Ministry of Education, Nanyang Technological University (NTU) and National University of Singapore (NUS). Hosted by NTU in partnership with NUS, SCELSE is linking new insights from the life sciences with expertise from emerging technologies in engineering and natural sciences to understand, harness and control microbial biofilm communities.

The microbial biofilms group is currently looking for a motivated and proactive Research Assistant to help undertake experiments investigating the interactions of mixed species biofilms with tissue culture cells. The team is involved in multi-disciplinary research across various fields and works very closely with the clinical research team and also external partners possessing diverse research expertise. 

The successful candidate for this position should be experienced in handling mammalian cell cultures and also possess skills for performing microbiological research. Experience with microbial-mammalian co-cultures would be an asset for this position. The candidate would work towards establishment of cell biology strategies to test the role of microbes and microbe related factors on cellular physiology. This is with an aim to decipher signatures unique to various phenotypes of interest to SCELSE and would involve working with an external industrial partner. The candidate would report to the microbiology project leader and is expected to be a team player with an active interest in carrying out inter-disciplinary research, particularly at the interface of Microbiology and Cell Biology.

Essential Skills:

  • Strong academic credentials - BSc in Biological Sciences
  • Ability to quickly learn and apply newly acquired knowledge
  • Demonstrated experience in handling and maintaining tissue culture cells, and the ability to grow and maintain bacteria and/or fungi
  • Experience in practical laboratory techniques
  • Active interest in inter-disciplinary research work
  • Good organisational skills and adaptability to regularly changing priorities and workloads
  • Effective communication skills and experience in collaborative research projects and inter-group communication

Interested applicants should submit curriculum vitae, a detailed letter of interest, and the names of three potential referees to SCELSE-HR@ntu.edu.sg. Please indicate ‘Research Assistant (Cell Culture and Microbiology)’ in the subject line.

 


Research Assistant (Nitric Oxide)

The Singapore Centre for Environmental Life Sciences Engineering (SCELSE) is a unique interdisciplinary Research Centre of Excellence (RCE), funded by National Research Foundation, Ministry of Education, Nanyang Technological University (NTU) and National University of Singapore (NUS). Hosted by NTU in partnership with NUS, SCELSE is linking new insights from the life sciences with expertise from emerging technologies in engineering and natural sciences to understand, harness and control microbial biofilm communities.

We are looking for a research assistant to work on a project investigating the use of nitric oxide (NO) to control biofilms formed by the opportunistic pathogen, Pseudomonas aeruginosa.  The individual will be responsible for establishing biofilm assays and for the primary screening of novel NO donors.  This work will include testing NO donors for release rates and determining release kinetics.  Further experiments will investigate the synergistic activity of NO donors in combination with standard antibiotics.  Safety profiles, based on tissue culture experiments will be performed and ultimately, the project will evaluate lead compounds in in vivo infection models. 

The candidate should have experience with biofilm formation, especially with P. aeruginosa.  Knowledge of and the ability to culture other species is desirable as is the ability to work with tissue culture cells.  Good organisational skills and record keeping, as well as attention to detail are a must.  Experience in microscopy is desirable.  Excellent English writing and communication skills are essential, as is the ability to work in a team based environment.

 Essential criteria:

  • BSc in Biology, Biotechnology or related discipline

  • Experience in culturing a range of bacteria including testing the growth of bacteria

  • Good organisational and communication skills

 Desirable criteria:

  • Microscopy experience

  • Tissue culture experience

Interested candidates should submit a current CV including potential referees and must submit a cover letter with the subject heading “Application for Research Assistant (Nitric Oxide)” to SCELSE-HR@ntu.edu.sg.