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Research

Research Assistant (Next-Gen Sequencing)

The Singapore Centre for Environmental Life Sciences Engineering (SCELSE) is a unique interdisciplinary Research Centre of Excellence (RCE), funded by National Research Foundation, Ministry of Education, Nanyang Technological University (NTU) and National University of Singapore (NUS). Hosted by NTU in partnership with NUS, SCELSE is linking new insights from the life sciences with expertise from emerging technologies in engineering and natural sciences to understand, harness and control microbial biofilm communities.

We are seeking to appoint a talented individual to join our team at SCELSE as a Research Assistant (Next-Gen Sequencing).

Key Responsibilities:

  • Quality control of incoming samples
  • DNA, RNA and amplicon library construction for various next-generation sequencing platforms
  • Operation of next-generation sequencing instruments and associated equipment
  • Troubleshooting and correction of suboptimal results under the guidance of scientific staff
  • Assistance in the improvement of existing protocols for sample preparation and library construction
  • Quantification of nucleic acids by real time PCR
  • DNA and RNA isolation from various sources
  • General laboratory and instrument maintenance

Requirements:

  • Bachelor’s degree in Biological Sciences or related field and/or combination of education and experience
  • Strong background in Genomics and Molecular Biology required
  • Solid knowledge and hands-on experience with molecular biological methods such as handling nucleic acids (DNA and RNA), enzymatic manipulation of nucleic acids, PCR and qPCR required
  • Hands-on experience with library preparation as well as the operation of next-generation sequencing platforms required
  • Hands-on experience with liquid handling platforms preferred
  • Desire and ability to learn and adapt to rapidly changing protocols and technologies
  • Ability to work independently as well as in a team setting
  • Excellent organisational, documentation and communication skills

Interested candidates should submit a current CV including potential references and submit a cover letter with the subject heading “Application for Research Assistant (Next-Gen Sequencing)” to SCELSE-HR@ntu.edu.sg.


Research Associate (Laboratory Automation)

The Singapore Centre for Environmental Life Sciences Engineering (SCELSE) is a unique interdisciplinary Research Centre of Excellence (RCE), funded by National Research Foundation, Ministry of Education, Nanyang Technological University (NTU) and National University of Singapore (NUS). Hosted by NTU in partnership with NUS, SCELSE is linking new insights from the life sciences with expertise from emerging technologies in engineering and natural sciences to understand, harness and control microbial biofilm communities.

We are seeking to appoint a talented individual to join our team at SCELSE as a Research Assistant (Laboratory Automation).

Key Responsibilities:

  • Develop new and improve existing protocols for nucleic acid extraction, NGS library preparation, PCR set-up and clean-up as well as qPCR set-up on our existing liquid handling platforms (Agilent, Tecan and Beckman Coulter)
  • Additional responsibilities include routine maintenance, calibration and verification of the automated platforms with the help of the manufacturers
  • Possibility of getting involved in manual and automated preparation of NGS libraries as well as the set-up of NGS sequencing runs on various sequencing platforms

Requirements:

  • Master’s degree in Biotechnology, Molecular Biology, Biochemistry, Engineering or closely related discipline and or combination of education and experience
  • Ability to develop application-specific protocols on various liquid handling platforms
  • Experience with operating and scripting liquid handling systems from Agilent, Tecan and/or Beckman Coulter is required
  • Experience with multiple computer programming languages preferred
  • Ability to troubleshoot and optimise performance of instrumentation
  • Fundamental knowledge of molecular biology protocols and practices, including nucleic acid extraction, purification and quantification, PCR and next-generation sequencing sample preparation workflows strongly preferred
  • Ability to work independently and prioritise tasks efficiently
  • Good general laboratory and organisational skills

Interested candidates should submit a current CV including potential references and submit a cover letter with the subject heading “Application for Research Associate (Laboratory Automation)” to SCELSE-HR@ntu.edu.sg.


Research Associate/Assistant (Air Microbiome/NAE)

The Singapore Centre for Environmental Life Sciences Engineering (SCELSE) is a unique interdisciplinary Research Centre of Excellence (RCE), funded by National Research Foundation, Singapore Ministry of Education, Nanyang Technological University and National University of Singapore. Hosted by NTU in partnership with NUS, SCELSE is linking new insights from the life sciences with expertise from the emerging technologies in engineering and natural sciences to understand, harness and control microbial biofilm communities.

SCELSE is seeking for a research assistant/associate to join us.

Main Responsibilities:

  • Isolation and quality-control of DNA and RNA from environmental samples
  • Genomic DNA extraction with high quality to subject to next generation sequencer
  • Troubleshooting and optimisation of existing DNA extraction protocols
  • Assisting with outdoor and indoor field sampling
  • Handling and maintaining cultures of microbial isolate

This position provides an opportunity to join a rapidly growing team of international researchers. For more information, visit http://scelse.sg/.

Requirements:

  • A university degree, Polytechnic diploma or extensive lab experience in Molecular Biology, Microbiology or related fields. Experience in molecular biology and microbiology lab for more than 6 months
  • General working knowledge of aseptic technique and basic molecular biological techniques
  • Familiarity with best laboratory practices and health & safety regulations
  • Experience of isolation and quality control of DNA and RNA samples for high throughput sequencing applications
  • Ability to work with minimal supervision
  • Excellent organisational skills, with the ability to manage and prioritise ongoing work
  • Ability to manage and maintain laboratory records and documentatio
  • Flexible approach to work and ability to work as part of a team
  • Familiarity with standard microbiological procedures for both general lab operations (e.g. microbiological media preparation) and experimental work (e.g. cultivation of microbes, especially environmental isolates)
  • Previous experience in high throughput sequencing pipelines would be beneficial
  • Experience with liquid handling robotics is an advantage
  • Ability to communicate in Chinese (Mandarin and other dialects) is a big plus

Interested candidates should submit a current CV including potential references and must submit a cover letter with the subject heading “Application for Research Associate/Assistant (Air Microbiome/NAE)” to SCELSE-HR@ntu.edu.sg.


Research Fellow (Bacteriology)  

The Singapore Centre for Environmental Life Sciences Engineering (SCELSE) is a unique interdisciplinary Research Centre of Excellence (RCE), funded by National Research Foundation, Singapore Ministry of Education, Nanyang Technological University and National University of Singapore. Hosted by NTU in partnership with NUS, SCELSE is linking new insights from the life sciences with expertise from the emerging technologies in engineering and natural sciences to understand, harness and control microbial biofilm communities.

We are seeking highly motivated individuals to work as Post-Doctoral researchers investigating host-pathogen interactions of Enterococcus faecalis and Streptococcus pneumoniae.  The candidate will work with an integrated team of microbiologists, immunologists, and cell biologists based in both Singapore and at the Karolinska Institutet in Stockholm, Sweden, to define structure-function correlates of these host-pathogen interactions. The project will include the application of in vitro microbiological and biochemical assays, cell-based and animal models of infection, high resolution imaging, analysis of changes in both host and pathogen gene and protein expression, and structural biology.

This project is a collaboration between the labs of Kimberly Kline and Birgitta Henriques-Normark. The work will be conducted within the Singapore Centre for Environmental Life Sciences Engineering (SCELSE), a Singapore Research Centre of Excellence. SCELSE has established a strong research platform for the investigation of functional microbial communities, in both environmental and infection contexts, with a specific focus on microbial biofilms. The centre is supported by its in-house ‘omics, bioinformatics and systems biology capacities, as well as a range of infection models and an in-house imaging facility.

Responsibilities

  • Work within a team to achieve key performance indicators of research project
    • Acquire proficiency in discipline-specific knowledge
    • Acquire new technical skills as required by the project
    • Plan, design and implement independent research project
    • Supervise research staff and/or graduate students in the planing, design, and implementation of research projects
    • Assist with organisation and management of the research team
  • Communicate research progress and outcomes regularly
    • Prepare technical reports for the programme PIs and present data at regular group meetings
    • Take lead role in manuscript preparation for scientific journals
    • Present research at external conferences and meetings
  • Maintain open communication with colleagues
    • Regular communication, as needed, with principal investigators, collaborating colleagues, lab members, Institutional Animal Care and Use Committee and Institutional Review Board, and SCELSE administrative staff
    • Serve as postdoctoral representative, on a limited basis, on internal committees

Requirements

  • PhD in Microbiology or related fields
  • Demonstrable expertise in handling microbial pathogens and in bacterial genetics
  • Demonstrable expertise in one or more of the following: Gram positive microbiology (particularly enterococci or pneumococci), protein biochemistry, structural biology, handling and analysis of ‘omics datasets, mammalian cell culture, murine models of infection
  • Strong track record of publication in relevant disciplines
  • Demonstrated ability to work in a multidisciplinary team
  • Good interpersonal and communication skills, oral and written, as well as organisational proficiency

Interested applicants should submit a cover letter describing their research experience, interests, and a curriculum vitae with the subject heading “Application for Research Fellow (Bacteriology)” to SCELSE-HR@ntu.edu.sg. Only short-listed candidates will be contacted.


Research Fellow (Bacterial Pathogenesis)

The Singapore Centre for Environmental Life Sciences Engineering (SCELSE) is a unique interdisciplinary Research Centre of Excellence (RCE), funded by Singapore’s National Research Foundation, Ministry of Education, Nanyang Technological University (NTU) and National University of Singapore (NUS). Hosted by NTU in partnership with NUS, SCELSE is linking new insights from the life sciences with expertise from emerging technologies in engineering, natural sciences and public health to understand, harness and control microbial biofilm communities.

We are seeking an individual to join us as a research fellow to study how Enterococcus faecalis interacts with mammalian cells during chronic biofilm-associated infections. The ideal candidate should have experience studying microbial interactions with mammalian cells. This project will involve mammalian cell culture, animal models of infection, imaging, and RNA and/or transposon sequencing experiments.

Requirements

  • PhD in microbiology, immunology, or cell biology
  • Experience in genetic manipulation of bacteria.
  • Experience in mammalian cell culture.
  • Experience in working with mice. 

Interested candidates should submit a current CV including potential references and must submit a cover letter with the subject heading “Application for Research Fellow (Bacterial Pathogenesis)” to SCELSE-HR@ntu.edu.sg.The cover letter should outline why the applicant is especially suited for this project and also discuss professional goals.


Research Fellow (SCELSE-NUS)

POSTDOCTORAL RESEARCH FELLOW POSITIONS AT NUS & SCELSE IN SINGAPORE.

The Singapore Centre for Environmental Life Sciences Engineering (SCELSE) is a unique interdisciplinary Research Centre of Excellence (RCE), funded by National Research Foundation, Ministry of Education, Nanyang Technological University (NTU) and National University of Singapore (NUS). Hosted by NTU in partnership with NUS, SCELSE is linking new insights from the life sciences with expertise from emerging technologies in engineering and natural sciences to understand, harness and control microbial biofilm communities.

1. Bacterial signalling mechanisms: Experience in bacterial molecular biology and genetics is necessary for this position. The chosen candidate will be part of an ongoing programme to unravel novel signalling mechanisms in bacteria for perception of biofilm-inducing volatile signals.

2. Environmental microbiomes of tropical urban freshwater systems: This ongoing programme combines multidisciplinary approaches in a highly active team to understand the drivers and functions of complex microbial communities in sedimentary and water phases, and rhizosphere of aquatic plants. A strong foundation in microbiology, biostatistics and ecology will be required.

3. Environmental microbiomes of tropical peatland systems: This ongoing programme combines multidisciplinary approaches in a highly active team to understand the drivers and functions of complex microbial communities in the peatlands of Southeast Asia. Work combines field and lab-based studies. A foundation in microbiology, biostatistics and ecology will be required.

4. Environmental and agricultural metabolomics: We are seeking an experienced mass spectrometry and analytical chemistry-trained postdoctoral candidate. The chosen candidate will join an existing long-running programme in metabolomics and a highly advanced mass spectrometry laboratory.

To apply, please contact Miko POH at dbsmpch@nus.edu.sg

For more information about the Metabolites Biology Lab, visit: https://smbl.nus.edu.sg