Research
SCELSE Imaging Facility

SCELSE has strong long-term collaborations with several international manufacturers of imaging platforms for the development and application of new technologies for real-time imaging of defined mixed-species and complex environmental biofilms, and microbiomes hosted in higher organisms, in robust reproducible experimental settings.

 

The SCELSE Advanced Biofilm Imaging Facility provides state-of-the-art instrumentation tailored to meet the highly specialised and continually evolving needs of interdisciplinary research for understanding and controlling environmental biofilms and microbiomes.

The facility’s instrumentation includes:

  • Leica TCS SP8 X WLL (white light laser) system
  • Zeiss Super Resolution System ELYRA PS.1 with LSM 780 system
  • Zeiss LSM 780 with 34 channels (32-GaAsP array detector + 2x PMTs)
  • Zeiss Axio Observer Z1-Inverted epifluorescence microscope
  • Zeiss Axio Imager M2-Upright epifluorescence microscope
  • Zeiss Lightsheet Z.1

The Leica TCS SP8-X WLL is the latest addition to the SCELSE Advanced Imaging Facility. It is a freely tunable confocal system with white light laser and add-on MatrixScreener extension for automated high-content screening.

 

The facility is also equipped with the ELYRA PS.1 (Palm-Structured Illumination) Superresolution Microscope LSM 780, and a LSM 780 confocal microscope.

The LSM 780 has an illumination and detection system allows simultaneous acquisition of up to ten dyes resulting in images of extreme detail, even from the most difficult specimens.

When coupled with the ELYRA PS.1, the LSM 780 systems provides a much higher resolution for all its images, localising small structures and even single molecules, achieving effective lateral resolutions down to 20 nm.

SCELSE is part of the Singapore Light Microscopy Facilities Network (SLMFN) which fosters interaction between the different microscope facilities in Singapore by providing a regular forum for staff to meet and network. Established by Dr. Graham Wright (IMB, A*STAR) the network is now coordinated by a committee. Meetings take place every 2 months at different venues across the island and are supported by host institutes and microscopy companies.

Please click HERE to go to the SLMFN website.