Senior Research Fellow

Dr Goran Biukovic is a senior research fellow in Asst Prof. Kimberly Kline’s research group. After the studies at the Food Science and Biotechnology faculty at the University of Zagreb, Croatia, he made his first research steps in the R&D institute of the pharmaceutical company Pliva Zagreb, where he was involved in drug research on polyketide antibiotics. The experience of genetics, molecular biology, and microbiology of the streptomycetes that he had gathered throughout his eight years of R&D research has helped him during his Ph.D. candidature at the Biology/Chemistry faculty at the University of Osnabrück, Germany. There, he isolated and characterised the enzymes involved in the biotransformation of antibiotic fusidic acid by streptomycetes. His first postdoc position was in the structural biology laboratory of Prof Grüber in School of Biological Sciences NTU, where he biochemically explored different proteins of the ATP synthase complex from various microorganisms (Archaea, Mycobacterium spp., Bacillus spp., yeast). These studies led his team to the discovery of a novel target site of a new antimycobacterial drug, bedaquiline in ATP synthase complex. His subsequent post doctoral position in Assoc Prof. Thomas Dick group in the Department of Microbiology and Immunology at NUS embarked on extensive research of bedaquiline-target interaction and the essentiality of different proteins of the ATP synthase complex in vivo, by combining recombineering and microbiological/biochemical analyses of generated mutants. In the Kline lab, he will develop new molecular tools to study Enterococcus spp. to understand the mechanisms of E. faecalis antagonism of  some organisms during mixed species interactions under different physiological and environmental conditions.