People
Mr Jean Pierre NSHIMYIMANA
PhD Student

Jean Pierre Nshimyimana

PhD Candidate, NTU & Singapore MIT-Alliance Fellow
Singapore-MIT Alliance for Research and Technology (SMART)
Singapore Center on Environmental Life Sciences Engineering (SCELSE)

 

Research Interest

Waterborne fecal contamination is associated with human health risk. Monitoring and source identification of fecal contamination in tropical environments pose a great challenge as fecal indicator bacteria developed and applied in temperate climates have been associated with environmental growth in the tropics. Tropical urban environments pose a greater challenge due to the presence of multiple potential sources of fecal microbial pollution.

My research is applying molecular biology tools (qPCR, digital PCR, & next generation sequencing) to contribute to addressing these challenges. I am interested in understanding:

1. How do bacterial communities and subgroups of sewage-associated taxa and pathogen-like bacteria vary as a function of land use, sites, and water quality in tropical urban catchments?

2. Can 16S rRNA host-associated Bacteroidales assays be used to effectively identify and distinguish non-point sources of fecal microbial pollution in urban tropical environments?

3. What indicators are appropriate for tracing pathogens from sewage contamination in tropical sediment-rich environments? Are sediments a reservoir, and potential source of fecal microbial pollution?

 

Journal papers:

Nshimyimana, J. P., Ekklesia, E., Shanahan, P., Chua, L. C. H., and Thompson, J. R. (2014). “Distribution and Abundance of Human Specific Bacteroides and Relation to Traditional Indicators in an Urban Tropical Catchment.” Journal of Applied Microbiology, 116 (5), pp. 1369 - 13683

Link: http://onlinelibrary.wiley.com/doi/10.1111/jam.12455/abstract

Tan,B., Ng, C. M., Nshimyimana, J.P., Loh, L.L., Gin, K.Y.H., and Thompson, J.R. (2015).” Next-generation sequencing (NGS) for assessment of microbial water quality: current progress, challenges, and future opportunities”. Frontiers in Microbiology,6, pp.1027

Link: http://journal.frontiersin.org/article/10.3389/fmicb.2015.01027/full

 

Conferences:

Recent: Poster Presentations

2015: American Society for Microbiology, New Orleans, USA

Nshimyimana, J. P., Freedman, A. J. E., Shanahan, P., Chua, L. C. H., and Thompson, J. R. (2015). “Variation of Bacterial Communities and Pathogen Taxa as a Function of Land Use and Water Quality in an Urban Tropical Catchment of Singapore” in Proceedings of the 115th General Meeting of American Society for Microbiology, New Orleans, USA.

Link: ftp://hydroanalysisinc.com/BWSC%20information/Nshimyimana2015.pdf

2015: International Water Association, Lisbon, Portugal

Nshimyimana, J. P., Freedman, A. J. E., Shanahan, P., Ekklesia, E., Chua, L. C. H., and Thompson, J. R. (2015). “Variation of Bacterial Communities and Human Pathogen-like Bacteria as a Function of Candidate Chemical Fecal Indicators and Fecal Indicator Bacteria in Tropical Urban Catchments of Singapore”. 18th International symposium on health-related water microbiology, Lisbon, Portugal. 

Recent: oral presentation

2014: UNC-Chapel Hill Water Institute, North Carolina, USA

Nshimyimana, J. P., Ekklesia, E., Shanahan, P., Chua, L. C. H., and Thompson, J. R. (2014). “Distribution and abundance of human specific Bacteroides and relation to traditional indicators in an urban tropical catchment, Singapore”. Water Microbiology Conference: Microbial contaminations from watersheds to human exposure, UNC-Chapel Hill, North Carolina, USA.