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Ms Sumitra Debina MITRA
PhD Student

Sumitra Debina Mitra
Ph.D candidate, NTU-SBS.

Supervisor: Asst. Prof. Kimberly A. Kline

Research Interest:

The role of Sortase A cytoplasmic tail in protein localization in Enterococcus faecalis.

Sortases are enzymes responsible for the sorting and covalent anchoring of surface proteins to the cell wall of Gram positive bacteria such as, E. faecalis. This microbe is a commensal and opportunistic pathogen responsible for a variety of nosocomial infections such as urinary tract infections, bacteremia, and wound infections. E. faecalis infections are difficult to treat due to increasing antibiotic resistance and there is a need for new drug targets. Sortases are now being viewed as potential targets due to their role in virulence factor expression on the cell wall. While the functionality of sortases are well understood, the exact mechanism of the enzymes’ focal localization and its’ role in mediating SecA localization during bacterial cell cycle is unclear. Our study aims to:

  1. Elucidate protein interacting partners that help mediate localization of SrtA and SecA
  2. Elucidate the functional residues of SrtA responsible for focal virulence factor assembly.

Journal papers:

Mitra, S.D., Afonina, I. and Kline, K.A. (2016) Right Place, Right Time: Focalization of Membrane Proteins in Gram-Positive Bacteria. Trends in Microbiology 24(8): 611-621.

Link: http://www.cell.com/trends/microbiology/abstract/S0966-842X(16)30001-4

Mitra, S., Kazi, M., Panchal, M., Rodrigues, C. and Shetty, A. (2015) Evaluation of Carba NP test for rapid detection of carbapenemase producing Enterobacteriaceae. Indian Journal of Medical Microbiology, 33(4), p.603.

Link: http://www.ijmm.org/text.asp?2015/33/4/603/167332