Mr TAN Jun Hou
PhD Student

Supervisor: Prof. Michael Givskov

Project Title: Discovery of anti-biofilm compounds through the targeting of cyclic-di-GMP regulatory pathway

Biofilms have been reported to be associated with majority of all bacteria infections. They have increased drug resistance resulting in chronic infections. Cyclic dimeric guanosine monophosphate (c-di-GMP) is a secondary messenger that was discovered to play a crucial role in the regulation of biofilm formation and motility. High level of intracellular c-di-GMP induces the formation of biofilm while low level of intracellular of c-di-GMP keeps the bacteria in their planktonic state. Since c-di-GMP signaling system is highly conversed among bacteria species and targeting c-di-GMP signaling system does not exert any bactericidal activity, we believe that c-di-GMP signaling system is an attractive target for the development of anti-biofilm compounds. The objective of Jun Hou’s research proposal is to identify and characterise c-di-GMP interference compounds that are able to inhibit biofilm formation via two-pronged approach: i) high throughput screening of existing synthetic small molecule libraries and ii) identification of novel anti-biofilm compounds using functional metagenomic approach.