Publications
Regulation of Burkholderia cenocepacia biofilm formation by RpoN and the c-di-GMP effector BerB
Reference: MicrobiologyOpen (2017): DOI: 10.1002/mbo1003.1480.

Knowledge of the molecular mechanisms that are involved in the regulation of biofilm formation is essential for the development of biofilm-control measures. It is well established that the nucleotide second messenger cyclic diguanosine monophosphate (c-di-GMP) is a positive regulator of biofilm formation in many bacteria, but more knowledge about c-di-GMP effectors is needed. We provide evidence that c-di-GMP, the alternative sigma factor RpoN (σ54), and the enhancer-binding protein BerB play a role in biofilm formation of Burkholderia cenocepacia by regulating the production of a biofilm-stabilising exopolysaccharide. Our findings suggest that BerB binds c-di-GMP, and activates RpoN-dependent transcription of the berA gene coding for a c-di-GMP-responsive transcriptional regulator. An increased level of the BerA protein in turn induces the production of biofilm-stabilising exopolysaccharide in response to high c-di-GMP levels. Our findings imply that the production of biofilm exopolysaccharide in B. cenocepacia is regulated through a cascade involving two consecutive transcription events that are both activated by c-di-GMP. This type of regulation may allow tight control of the expenditure of cellular resources.

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Published By
Fazli M., Rybtke M., Steiner E., Weidel E., Berthelsen J., Groizeleau J., Bin W., Zhi B.Z., Yaming Z., Kaever V., Givskov M., Hartmann R.W., Eberl L., Tolker-Nielsen T.