Search Publications

Solid-phase synthesis and biological evaluation of piperazine-based novel bacterial topoisomerase inhibitors

by Flagstad T., Pedersen M. T., Jakobsen T. H., Felding J., Tolker-Nielsen T., Givskov M., Qvortrup K., Nielsen T.E.

Reference: Bioorganic & Medicinal Chemistry Letters (2022) 57: 128499.

Research Cluster(s): Biofilm Biology

Programme(s): Biofilm Detection and Control

Adaptation to an amoeba host leads to Pseudomonas aeruginosa isolates with attenuated virulence

by Leong W., Poh W. H., Williams J. , Lutz C., Hoque M. M., Poh Y. H., Yee B. Y. K., Chua C., Givskov M., Sanderson-Smith M., Rice S. A., McDougald D.

Reference: Applied and Environmental Microbiology (2022): doi/10.1128/aem.02322-21

Research Cluster(s): Biofilm Biology

Programme(s): Host Microbiome (Holobiont) Interactions

SAR study of 4-arylazo-3,5-diamino-1H-pyrazoles: identification of small molecules that induce dispersal of Pseudomonas aeruginosa biofilms

by Jansen C. U., Uhd J., Andersen J. B., Hultqvist L. D., Jakobsen T. H., Nilsson M., Nielsen T.E., Givskov M., Tolker-Nielsen T., Qvortrup K. M.

Reference: RSC Med Chem (2021) 12: 1868-1878

Research Cluster(s): Biofilm Biology

Programme(s): Biofilm Detection and Control

Transcriptional activity of predominant Streptococcus species at multiple oral sites associate with periodontal status

by Belstrøm D., Constancias F., Markvart M., Sikora M., Sørensen C. E., Givskov M.

Reference: Frontiers in Cellular and Infection Microbiology (2021) 11: 752664.

Research Cluster(s): Biofilm Biology

Programme(s): Host Microbiome (Holobiont) Interactions Public Health & Medicine

Periodontitis associates with species-specific gene expression of the oral microbiota

by Belstrøm D., Constancias F., Moses D., Schuster S. C., Mark VELEBA, Mahé F., Givskov M.

Reference: npj Biofilms and Microbiomes (2021) 7: 76

Research Cluster(s): Biofilm Biology Meta-'omics & Microbiomes

Programme(s): Public Health & Medicine

Carbon starvation of Pseudomonas aeruginosa biofilms selects for dispersal insensitive mutants

by H. Nair, Subramoni, Poh W. H., Nabilah Taqiah Binte HASNUDDIN, Tay Q. X. M., Givskov M., , Kjelleberg S., McDougald D., Rice S. A.

Reference: BMC Microbiology (2021) 21: 255

Research Cluster(s): Biofilm Biology

Programme(s): Experimental Biofilms & Synthetic Ecology

Disruption of the Pseudomonas aeruginosa Tat system perturbs PQS-dependent quorum sensing and biofilm maturation through lack of the Rieske cytochrome bc1 sub-unit

by Soh E.Y., Smith F., Gimenez M.R., Yang L., Vejborg R.M., Fletcher M., Halliday N., Bleves S., Heeb S., Cámara M., Hardie K.R., Givskov M., Tolker-Nielsen T., Ize B., Williams P.

Reference: PLoS Pathogens (2019) 17: e1009425

Research Cluster(s): Biofilm Biology

Programme(s): Biofilm Detection and Control

Identification of small molecules that interfere with c-di-GMP signaling and induce dispersal of Pseudomonas aeruginosa biofilms

by Andersen J.B., Hultqvist L.D., Jansen C.U., Jakobsen T.H., Nilsson M., Rybtke M., Uhd J., Fritz B.G., Seifert R., Berthelsen J., Nielsen T.E., T.E., Givskov M., Qvortrup K.

Reference: npj Biofilms and Microbiomes (2021) 7: 59

Research Cluster(s): Biofilm Biology

Programme(s): Biofilm Detection and Control

Induction of native c-di-GMP phosphodiesterases leads to dispersal of Pseudomonas aeruginosa biofilms

by Andersen J.B., Kragh K.N., Hultqvist L.D., Rybtke M., Nilsson M., Jakobsen T.H., Givskov M., Tolker-Nielsen T.

Reference: Antimicrobial Agents and Chemotherapy (2021) 64: e02431-20.

Research Cluster(s): Biofilm Biology

Programme(s): Biofilm Detection and Control

Selectivity of kinase inhibitors

by Wu P., Givskov M., Nielsen T.E.

Reference: Wu P., Givskov M. and Nielsen T. E. (2017). Selectivity of kinase inhibitors. Drug Selectivity: An Evolving Concept in Medicinal Chemistry (vol. 72). H. Buschmann and N. Handler. Weinheim, Germany, Wiley-VCH Verlag GmbH & Co. KGaA.

Research Cluster(s): Biofilm Biology

Programme(s): Biofilm Detection and Control

Small molecule anti-biofilm agents developed on the basis of mechanistic understanding of biofilm formation

by Qvortrup K., Hultqvist L.D., Nilsson M., Jakobsen T.H., Jansen C.U., Uhd J., Andersen J.B., Nielsen T.E., Givskov M.,

Reference: Frontiers in Chemistry (2019) 7: 742-742

Research Cluster(s): Biofilm Biology

Programme(s): Biofilm Detection and Control

Inactivation of the pgmA gene in Streptococcus mutans significantly decreases biofilm-associated antimicrobial tolerance

by Nilsson M., Givskov M., Twetman S., Tolker-Nielsen T.

Reference: Microorganisms (2019 )7: 310

Research Cluster(s): Biofilm Biology

Programme(s): Biofilm Detection and Control

Reactivity and synthetic applications of multicomponent petasis reactions

by Wu P., Givskov M., Nielsen T.E.

Reference: Chemical Reviews (2019) 119: 11245-11290

Research Cluster(s): Biofilm Biology

Programme(s): Biofilm Detection and Control

Visualizing biofilm by targeting eDNA with long wavelength probe CDr15

by Kwon H.-Y., Kim J.-Y., Liu X., Lee J.Y., Yam K. H. J., Dahl Hultqvist L., Xu W., Rybtke M., Tolker-Nielsen T., Heo W., Kim J.-J., Kang N.-Y., Joo T., Yang L., Park S.-J., Givskov M., Chang Y.

Reference: Biomaterials Science (2019) 7: 3594-3598

Research Cluster(s): Biofilm Biology

Programme(s): Biofilm Detection and Control

Transposon mutagenesis in Streptococcus species

by Nilsson M., Givskov M., Tolker-Nielsen T.

Reference: Methods in Molecular Biology (2019) 2016: 39-49

Research Cluster(s): Biofilm Biology

Increased intracellular cyclic di-AMP levels sensitize Streptococcus gallolyticus to osmotic stress and reduce biofilm formation and adherence on intestinal cells

by Teh W. K. , Dramsi S., Tolker-Nielsen T., Yang L., Givskov M.

Reference: Journal of Bacteriology (2019) 201: e00597-00518

Research Cluster(s): Biofilm Biology

Programme(s): Biofilm Detection and Control

Key players and individualists of cyclic-di-GMP signaling in Burkholderia cenocepacia

by Richter A.M., Fazli M., Schmid N., Shilling R., Suppiger A., Givskov M., Eberl L., Tolker-Nielsen T.

Reference: Frontiers in Microbiology (2019) 9: 3286

Research Cluster(s): Biofilm Biology

Programme(s): Biofilm Detection and Control

High levels of cAMP inhibit Pseudomonas aeruginosa biofilm formation through reduction of the c-di-GMP content

by Almblad H., Rybtke M., Hendiani S., Andersen J. B., Givskov M., Tolker-Nielsen T.

Reference: Microbiology (2019) 165: 324-333

Research Cluster(s): Biofilm Biology

Programme(s): Biofilm Detection and Control Public Health & Medicine

Oxidative stress response plays a role in antibiotic tolerance of Streptococcus mutans biofilms

by Nilsson M., Jakobsen T.H., Givskov M., Twetman S., Tolker-Nielsen T.

Reference: Microbiology (2019) 165: 334-342

Research Cluster(s): Biofilm Biology

Programme(s): Biofilm Detection and Control

Itaconimides as novel quorum sensing inhibitors of Pseudomonas aeruginosa

Reference: Frontiers in Cellular and Infection Microbiology (2019) 8: 443-443

Repurposing the anticancer drug cisplatin with the aim of developing novel Pseudomonas aeruginosa infection control agents

by Yuan M., Chua S. L., Liu Y., Moses D., Yam K. H. J., Aung T. T., Beuerman R. W., Salido M. M. S., Schuster S. C., Tan C.-H., Givskov M., Yang L., Nielsen T. E

Reference: Beilstein Journal of Organic Chemistry (2018) 14: 3059-3069

Research Cluster(s): Biofilm Biology Meta-'omics & Microbiomes

Programme(s): Biofilm Detection and Control

Matrix polysaccharides and SiaD diguanylate cyclase alter community structure and competitiveness of Pseudomonas aeruginosa during dual-species biofilm development with Staphylococcus aureus

by Chew S. C., Chew S.C., Yam K. H. J., Matysik A. S., Seng Z.J., , Givskov M., Givskov M., Rice S. A., Yang L., Kjelleberg S.

Reference: mBio (2018) 9: e00585-00518

Research Cluster(s): Biofilm Biology

CDy14: a novel biofilm probe targeting exopolysaccharide Psl

by Kwon H.-Y., Kim J.-Y., Lee J.Y., Yam K. H. J., J.K.H., , Hultqvis, L.D., Xu W., Rybtke M., Yang L., Kim J.-J., Givskov M., Chang Y.

Reference: Chemical Communications (2020) 54: 11865-11868

Research Cluster(s): Biofilm Biology

Programme(s): Biofilm Detection and Control Public Health & Medicine

Interleukin-26 (IL-26) is a novel anti-microbial peptide produced by T cells in response to staphylococcal enterotoxin.

by Woetmann A., Alhede M., Dabelsteen S., Bjarnsholt T., Rybtke M., Nastasi C., Krejsgaard T., Andersen M. H., Bonefeld C. M., Geisler C., Givskov M., Odum N.

Reference: Oncotarget (2018) 9: 19481–19489

Research Cluster(s): Biofilm Biology

Programme(s): Biofilm Detection and Control

Combination therapy strategy of quorum quenching enzyme and quorum sensing inhibitor in suppressing multiple quorum sensing pathways of P. aeruginosa

by Fong J., Zhang C., Yang R., Boo Z. Z., Tan S. K., Nielsen T.E., Givskov M., Liu X.-W., Bin W., Su H., Yang L.

Reference: Scientific Reports (2018) 8: 1155.

Research Cluster(s): Biofilm Biology

Programme(s): Biofilm Detection and Control

Imaging N-acyl homoserine lactone quorum sensing in vivo

by Jakobsen T.H., Alhede M., Hultqvist L.D., Bjarnsholt T., Givskov M.

Reference: Methods in Molecular Biology (2017), vol 1673. Humana Press, New York, NY

Research Cluster(s): Biofilm Biology

Programme(s): Biofilm Detection and Control

Qualitative and quantitative determination of quorum sensing inhibition in vitro

by Jakobsen T.H., Alhede M., Hultqvist L.D., Bjarnsholt T., Givskov M.

Reference: Quorum Sensing. Methods in Molecular Biology. L. Leoni and G. Rampioni. New York, NY, Humana Press, New York (2018) Vol 1673: 275-285.

Research Cluster(s): Biofilm Biology

Programme(s): Developmental Biology of Biofilms Biofilm Detection and Control

Reduced intracellular c-di-GMP content increases expression of quorum sensing-regulated genes in Pseudomonas aeruginosa

by Chua S.L., Liu Y., Li Y., Ting H.J., Kohli G.S., Cai Z., Suwanchaikasem P., Goh K.K.K., Ng S.P., Tolker-Nielsen T., Yang L., Givskov M.

Reference: Frontiers in Cellular and Infection Microbiology (2017) 7: doi: 10.3389/fcimb.2017.00451

Research Cluster(s): Biofilm Biology

Programme(s): Developmental Biology of Biofilms Biofilm Detection and Control

Metagenomic and metatranscriptomic analysis of saliva reveals disease-associated microbiota in patients with periodontitis and dental caries

by Belstrøm D., Constancias F., Liu Y., Yang L., Moses D., Schuster S. C., Kohli G.S., Jakobsen T.H., Holmstrup P., Givskov M.

Reference: npj Biofilms and Microbiomes (2017) 3: 23.

Research Cluster(s): Biofilm Biology Meta-'omics & Microbiomes

Programme(s): Host Microbiome (Holobiont) Interactions

Bacterial biofilm control by perturbation of bacterial signaling processes

by Jakobsen T.H., Tolker-Nielsen T., Givskov M.

Reference: International Journal of Molecular Sciences (2017) 18: 1970.

Research Cluster(s): Biofilm Biology

Programme(s): Developmental Biology of Biofilms Biofilm Detection and Control

A broad range quorum sensing inhibitor working through sRNA inhibition

by Jakobsen T.H., Warming A.N., Vejborg R.M., Moscoso J.A., Stegger M., Lorenzen F., Rybtke M., Andersen J.B., Petersen R., Andersen P.S., Nielsen T.E., Tolker-Nielsen T., Filloux A., Ingmer H., Givskov M.

Reference: Scientific Reports (2017) 7: 9857.

Research Cluster(s): Biofilm Biology

Programme(s): Developmental Biology of Biofilms Biofilm Detection and Control

The acquisition of resistance to carbapenem and macrolide-mediated quorum sensing inhibition by Pseudomonas aeruginosa via a novel integrative and conjugative element ICETn43716385

by Ding Y., Teo J., Moses D., Schuster S. C., Givskov M., Yang L.

Reference: bioRxiv (2017) doi: https://doi.org/10.1101/161497

Research Cluster(s): Biofilm Biology Meta-'omics & Microbiomes

Programme(s): Biofilm Detection and Control

Discovery of novel antimycobacterial drug therapy in biofilm of pathogenic nontuberculous mycobacterial keratitis

by Aung T.T, Chor W.H.J, Yam K. H. J., Givskov M., Yang L., Beuerman R.W.

Reference: The Ocular Surface: http://dx.doi.org/10.1016/j.jtos.2017.06.002

Research Cluster(s): Biofilm Biology

Programme(s): Biofilm Detection and Control

High-throughput screening for compounds that modulate the cellular c-di-GMP level in bacteria

by Groizeleau J. , Andersen J. B. , Givskov M., Berthelsen J., Tolker-Nielsen T.

Reference: Methods in Molecular Biology (2017) 1657: 455-470.

Research Cluster(s): Biofilm Biology

Programme(s): Biofilm Detection and Control

Regulation of Burkholderia cenocepacia biofilm formation by RpoN and the c-di-GMP effector BerB

by Fazli M., Rybtke M., Steiner E., Weidel E., Berthelsen J., Groizeleau J., Bin W., Zhi B.Z., Yaming Z., Kaever V., Givskov M., Hartmann R.W., Eberl L., Tolker-Nielsen T.

Reference: MicrobiologyOpen (2017): DOI: 10.1002/mbo1003.1480.

Research Cluster(s): Biofilm Biology

Programme(s): Biofilm Detection and Control

Fusaric acid and analogues as Gram-negative bacterial quorum sensing inhibitors

by Tung T. T., Jakobsen T. H., Dao T. T., Fuglsang A. T., Givskov M., Christensen S. B., Nielsen J.

Reference: European Journal of Medicinal Chemistry (2017) 126: 1011-1020

Research Cluster(s): Biofilm Biology

Programme(s): Biofilm Detection and Control

Disulfide bond-containing ajoene analogues as novel quorum sensing inhibitors of Pseudomonas aeruginosa

by Fong J., Yuan M., Jacobsen T. H., Mortensen K. T., Delos Santos M. M. S., Chua S. L., Yang L., Tan C. H., Nielsen T.E., Givskov M.

Reference: Journal of Medicinal Chemistry (2017) 60: 215-227

Research Cluster(s): Biofilm Biology

Programme(s): Biofilm Detection and Control

Cyclic-di-GMP is required for corneal infection by Pseudomonas aeruginosa and modulates host immunity

by Yam K. H. J., Aung T. T., Chua S. L., Chen Y., Kohli G.S., Zhou J., Constancias F., Liu Y., Cai Z., Salido S.S., Moses D., Rice S. A., Schuster S. C., Wu B., Kjelleberg S., Tolker-Nielsen T., Bueurman R. W., Givskov M., Yang L.

Reference: bioRxiv (2017): doi.org/10.1101/098749

Research Cluster(s): Biofilm Biology Meta-'omics & Microbiomes

Programme(s): Host Microbiome (Holobiont) Interactions Biofilm Detection and Control

Reactive oxygen species drive evolution of pro-biofilm variants in pathogens by modulating cyclic-di-GMP levels

by Chua S. L., Ding Y., Liu Y., Cai Z., Swarup S., Moses D., Schuster S. C., Kjelleberg S., Givskov M., Yang L.

Reference: Open Biology (2016) 6(11) DOI: 10.1098/rsob.160162

Research Cluster(s): Biofilm Biology Environmental Engineering Meta-'omics & Microbiomes

Programme(s): Developmental Biology of Biofilms Biofilm Detection and Control

The anti-cancerous drug doxorubicin decreases the c-di-GMP content in Pseudomonas aeruginosa but promotes biofilm formation

by Groizeleau J., Rybtke M., Andersen J. B., Berthelsen J., Liu Y., Yang L., Nielsen T.E., Kaever E., Givskov M., Tolker-Nielsen T

Reference: Microbiology (2016) 162: 1797-1807.

Research Cluster(s): Biofilm Biology

Selective labelling and eradication of antibiotic-tolerant bacterial populations in Pseudomonas aeruginosa biofilms

by Chua S. L., Yam K. H. J., Hao P., Adav S.S., Salido M.M., Liu Y., Givskov M., Sze S.K., Tolker-Nielsen T., Yang L.

Reference: Nature Communications (2016) 7: 10750.

Research Cluster(s): Biofilm Biology

Absence of bacteria on coronary angioplasty balloons from unselected patients: Results with use of a high sensitivity polymerase chain reaction assay

by Hansen G.M., Nilsson M., Nielsen C.H., Holmstrup P., Helqvist S., Tolker-Nielsen T., Givskov M., Hansen P.R.

Reference: PLoS ONE (2015) 10: e0145657.

Research Cluster(s): Biofilm Biology

Programme(s): Host Microbiome (Holobiont) Interactions

Detection of pathogenic biofilms with bacterial amyloid targeting fluorescent probe, CDy11

by Kim J.-Y., Sahu S., Yau Y.-H., Wang X., Shochat S.G., Nielsen P. H., Dueholm M.S., Otzen D.E., Lee J., Delos Santos M.M.S., Yam K. H. J., Kang N.-Y., Park S.-J., Kwon H., Seviour T. W., Yang L., Givskov M., Chang Y.-T.

Reference: Journal of the American Chemical Society (2016) 138: 402-407

Research Cluster(s): Biofilm Biology

RpoN regulates virulence factors of Pseudomonas aeruginosa via modulating the PqsR quorum sensing regulator

by Cai Z., Liu Y., Chen Y., Yam K. H. J., Chew S.C., Chua S. L., Wang K., Givskov M., Yang L.

Reference: International Journal of Molecular Sciences (2015) 16: 28311-28319.

Research Cluster(s): Biofilm Biology

Programme(s): Developmental Biology of Biofilms Biofilm Detection and Control

Spatially extensive microbial biogeography of the Indian Ocean provides insights into the unique community structure of a pristine coral atoll

by T.C. Jeffries, M. Ostrowski, Williams R. B. H., C. Xie, R.M. Jensen , J.J. Grzymski, S.J. Senstius, Givskov M., R. Hoeke, G.K. Philip, R. Y. Neches, Moses D., Chénard C., I.T. Paulsen, Lauro F. M.

Reference: Scientific Reports (2015) 5: 15383.

Research Cluster(s): Biofilm Biology Meta-'omics & Microbiomes

Biofilms of pathogenic nontuberculous mycobacteria targeted by new therapeutic approaches

by Aung T.T., Yam K. H. J., Lin S., Salleh S.M., Givskov M., Liu S., Lwin N.C., Yang L., Beuerman R.W.

Reference: Antimicrobial Agents and Chemotherapy (2016) 60: 24-35.

Research Cluster(s): Biofilm Biology

Chemical biology strategies for biofilm control

by Yang L., Givskov M.

Reference: Yang L. and Givskov M. (2015). Chemical biology strategies for biofilm control. In: M. Ghannoum, M. Parsek, M. Whiteley and P. Mukherjee (eds) Microbial Biofilms, 2nd Ed. Washington DC, ASM Press: pp. 363-372.

Research Cluster(s): Biofilm Biology

Programme(s): Host Microbiome (Holobiont) Interactions Biofilm Detection and Control

In vitro and in vivo generation and characterization of Pseudomonas aeruginosa biofilm-dispersed cells via c-di-GMP manipulation

by Chua S. L., Hultqvist L.D., Yuan M., Rybtke M., Nielsen T.E., Givskov M., Tolker-Nielsen T., Yang L.

Reference: Nature Protocols (2015) 10: 1165-1180.

Research Cluster(s): Biofilm Biology

Programme(s): Developmental Biology of Biofilms Biofilm Detection and Control

Antibiofilm properties of acetic acid

by Bjarnsholt, Thomas , Alhede, Morten , Jensen, Peter Ostrup , Nielsen, Givskov M., Johansen

Reference: Advances in Wound Care (2015) 4: 363-372.

Research Cluster(s): Biofilm Biology

Programme(s): Host Microbiome (Holobiont) Interactions Biofilm Detection and Control

Complete genome sequence and transcriptomic analysis of the novel pathogen Elizabethkingia anophelis in response to oxidative stress

by Li Y., Liu Y., Chew S.C., Tay Q. X. M., Salido S.S., Teo J., Lauro F. M., Givskov M., Yang L.

Reference: Genome Biology and Evolution (2015) 7(6): 1676-1685.

Research Cluster(s): Biofilm Biology Meta-'omics & Microbiomes

C-di-GMP regulates Pseudomonas aeruginosa stress response to tellurite during both planktonic and biofilm modes of growth

by Chua S. L., Sivakumar K., Morten Yuan, Yuan M., Jens Bo Nielsen, Nielsen T.E., Givskov M., Tim Cao, Cao B., Kjelleberg S., Yang L.

Reference: Scientific Reports (2015) 5: 10052.

Research Cluster(s): Biofilm Biology

Programme(s): Developmental Biology of Biofilms Biofilm Detection and Control

Synthesis of substituted y- and d-lactams through mannich-type reactions of solid-supported N-acyliminium ions

by Komnatnyy V.V., Taveras K.M., Nandurkar N.S., Le Quement S.T., Givskov M., Nielsen T.E.

Reference: European Journal of Organic Chemistry (2015) 16: 3524-3530.

Research Cluster(s): Biofilm Biology

Programme(s): Bioprocessing Biofilm Detection and Control

Multiple diguanylate cyclase-coordinated regulation of pyoverdine synthesis in Pseudomonas aeruginosa

by Chen Y., Yuan M., Mohanty A., Yam K. H. J., Liu Y., Chua S. L., Nielsen T.E., Tolker-Nielsen, Givskov M., Cao B., Yang L.

Reference: Environmental Microbiology Reports (2015) 7(3): 498-507

Research Cluster(s): Biofilm Biology

Programme(s): Bioprocessing Biofilm Detection and Control

Triazole-containing N-acyl homoserine lactones targeting the quorum sensing system in Pseudomonas aeruginosa

by Hansen M.R., Jakobsen T.H., Bang C.G., Cohrt A.E., Hansen C.L., Clausen J.W., Le Quement S.T., Tolker-Nielsen T., Givskov M., Nielsen T.E.

Reference: Bioorganic & Medicinal Chemistry (2015) 23: 1638-1650.

Research Cluster(s): Biofilm Biology

Programme(s): Biofilm Detection and Control

Autofluorescence in samples obtained from chronic biofilm infections – "all that glitters is not gold"

by Eickhardt S., Kragh K.N., Schroder S., Poulsen S., Seier Sillesen H., Givskov M., Hoiby N., Bjarnsholt T., Alhede M.

Reference: Pathogens and Disease (2015) 73: ftv012.

Research Cluster(s): Biofilm Biology

Programme(s): Host Microbiome (Holobiont) Interactions Biofilm Detection and Control

Functional amyloids keep quorum sensing molecules in check

by Seviour T. W., Hansen S.H., Liu Y., Yau Y.H., Wang V. B., Stenvang M.R., Christiansen G., Marsili E., Givskov M., Chen Y., Otzen D.E., Nielsen P. H., Shochat S.G., Kjelleberg S., Dueholm M.S.

Reference: Journal of Biological Chemistry (2015) 290(10): 6457-6469

Research Cluster(s): Biofilm Biology

Programme(s): Bioprocessing

Combining the petasis 3-component reaction with multiple modes of cyclization: A build/couple/pair strategy for the synthesis of densely functionalized small molecules

by Flagstad T., Hansen M. R., Le Quementt S. T., Givskov M., Nielsen T.E.

Reference: ACS Combinatorial Science (2015) 17: 19-23.

Research Cluster(s): Biofilm Biology

Synthesis of 4-halogenated 3-fluoro-6-methoxyquinolines: Key building blocks for the synthesis of antibiotics

by Flagstad T., Petersen M.T., Hinnerfeldt D.M., Givskov M., Nielsen T.E.

Reference: Synthesis-Stuttgart (2014) 46(23): 3263-3267

The Pseudomonas aeruginosa type iii translocon is required for biofilm formation at the epithelial barrier

by Tran C.S., Rangel S.M., Almblad H., Kierbel A., Givskov M., Tolker-Nielsen T., Hauser A.R., Engel J.N.

Reference: PLoS Pathogens (2014) 10(11): 11

Population dynamics of an Acinetobacter baumannii clonal complex during colonization of patients

by Wen H., Wang K., Liu Y., Tay Q. X. M., Lauro F. M., Huang H., Wu H., Liang H., Ding Y., Givskov M., Chen Y., Yang L.

Reference: Journal of Clinical Microbiology (2014) 52(9): 3200-3208

Research Cluster(s): Biofilm Biology

Comparative systems biology analysis and mode of action of the isothiocyanate compound iberin on Pseudomonas aeruginosa

by Tan S.Y., Liu Y., Chua S. L., Vejborg R.M., Jakobsen T.H., Chew S.C., Li Y., Nielsen T.E., Tolker-Nielsen T., Yang L., Givskov M.

Reference: Antimicrobial Agents and Chemotherapy (2014) 58(11): 6648-6659

Research Cluster(s): Biofilm Biology

Dispersed cells represent a distinct stage in the transition from bacterial biofilm to planktonic lifestyles

by Chua S. L., Liu Y., Yam K. H. J., Chen Y., Vejborg R.M., Tan B.G.C., Kjelleberg S., Tolker-Nielsen T., Givskov M., Yang L.

Reference: Nature Communications (2014) 5: 4462

Research Cluster(s): Biofilm Biology

Programme(s): Developmental Biology of Biofilms

Regulation of biofilm formation in Pseudomonas and Burkholderia species

by Fazli M., Almblad H., Rybtke M.L., Givskov M., Eberl L., Tolker-Nielsen T.

Reference: Environmental Microbiology (2014) 16(7): 1961-1981

Programme(s): Bioprocessing Developmental Biology of Biofilms

A mariner transposon vector adapted for mutagenesis in oral streptococci

by Nilsson M., Christiansen N., Hoiby N., Twetman S., Givskov M., Tolker-Nielsen T.

Reference: MicrobiologyOpen (2014) 3(3): 333-340

Programme(s): Host Microbiome (Holobiont) Interactions Biofilm Detection and Control

Comparative genomic analysis of malaria mosquito vector-associated novel pathogen Elizabethkingia anophelis

by Teo J., Tan S.Y., Liu Y., Tay Q. X. M., Ding Y., Li Y., Kjelleberg S., Givskov M., Lin R.T.P., Yang L.

Reference: Genome Biology and Evolution (2014) 6(5): 1158-1165

Research Cluster(s): Biofilm Biology

Effects of Radix Ginseng on microbial infections: A narrative review

by Wu H., Hoiby N., Yang L., Givskov M., Song Z.J.

Reference: Journal of Traditional Chinese Medicine (2014) 34(2): 227-233

Research Cluster(s): Biofilm Biology

Bacterial biofilm formation and treatment in soft tissue fillers

by Alhede M., Er O., Eickhardt S., Kragh K., Alhede M., Christensen L.D., Poulsen S.S., Givskov M., Christensen L.H., Hoiby N., Tvede M., Bjarnsholt T.

Reference: Pathogens and Disease (2014) 70(3): 339-346

Draft genome sequence of the model naphthalene-utilizing organism Pseudomonas putida OUS82

by Tay Q. X. M., Roizman D., Cohen Y., Tolker-Nielsen T., Givskov M., Yang L.

Reference: Genome Announcements (2014) 2(1): e01161-01113

Research Cluster(s): Biofilm Biology

Emerging frontiers in detection and control of bacterial biofilms

by Tan Y. E. S., Chew S.C., Tan S.Y., Givskov M., Yang L.

Reference: Current Opinion in Biotechnology (2014) 26: 1-6

Research Cluster(s): Biofilm Biology

Programme(s): Biofilm Detection and Control

The common oceanographer: Crowdsourcing the collection of oceanographic data

by Lauro F. M., Senstius S.J., Cullen J., Neches R., Jensen R.M., Brown M.V., Darling A.E., Givskov M., McDougald D., Hoeke R., Ostrowski M., Philip G.K., Paulsen I.T., Grzymski J.J.

Reference: PLoS Biol (2014) 12(9): e1001947

Research Cluster(s): Biofilm Biology Meta-'omics & Microbiomes

Bacteria-triggered release of antimicrobial agents

by Chiang W.-C., Tolker-Nielsen T., Nielsen T.E., Givskov M., Komantnyy V.V.

Reference: Angewandte Chemie (2014) 126(2): 449-451

Research Cluster(s): Biofilm Biology

Chapter One - Pseudomonas aeruginosa biofilms: Mechanisms of immune evasion

by Alhede M., Bjarnsholt T., Givskov M., Alhede M.

Reference: Advances in Applied Microbiology. S. Sima and M. G. Geoffrey, Academic Press (2014). Volume 86: 1-40

Research Cluster(s): Biofilm Biology

Programme(s): Biofilm Detection and Control

Identification of five structurally unrelated quorum-sensing inhibitors of Pseudomonas aeruginosa from a natural-derivative database

by Tan S.Y., Chua S. L., Chen Y., Rice S. A., Kjelleberg S., Nielsen T.E., Yang L., Givskov M.

Reference: Antimicrobial Agents and Chemotherapy (2013) 57(11): 5629-5641

Research Cluster(s): Biofilm Biology

Programme(s): Biofilm Detection and Control

Applying insights from biofilm biology to drug development - can a new approach be developed?

by Bjarnsholt T., Ciofu O., Molin S., Givskov M., Hoiby N.

Reference: Nature Reviews Drug Discovery (2013) 12(10): 791-808

Research Cluster(s): Biofilm Biology

Programme(s): Biofilm Detection and Control

Clearance of Pseudomonas aeruginosa foreign-body biofilm infections through reduction of the cyclic di-GMP level in the bacteria

by Christensen L.D., van Gennip M., Rybtke M.T., Wu H., Chiang W.C., Alhede M., Høiby N., Nielsen T.E., Givskov M., Tolker-Nielsen T.

Reference: Infection and Immunity (2013) 81(8): 2705-2713

Engineering PQS biosynthesis pathway for enhancement of bioelectricity production in Pseudomonas aeruginosa microbial fuel cells

by Wang V. B., Chua S. L., Cao B., Seviour T. W., Nesatyy V.J., Marsili E., Kjelleberg S., Givskov M., Tolker-Nielsen T., Song H., Loo J. S. C., Yang L.

Reference: PLoS ONE (2013) 8(5): e63129

Research Cluster(s): Biofilm Biology Environmental Engineering

Bis-(3'-5')-cyclic dimeric GMP regulates antimicrobial peptide resistance in Pseudomonas aeruginosa

by Chua S. L., Tan S.Y., Rybtke M.T., Chen Y., Rice S. A., Kjelleberg S., Tolker-Nielsen T., Yang L., Givskov M.

Reference: Antimicrobial Agents and Chemotherapy (2013) 57(5): 2066-2075

Research Cluster(s): Biofilm Biology

Programme(s): Biofilm Detection and Control

Comparative transcriptomic analysis of the Burkholderia cepacia tyrosine kinase bceF mutant reveals a role in tolerance to stress, biofilm formation, and virulence

by Ferreira A.S., Silva I.N., Oliveira V.H., Becker J.D., Givskov M., Ryan R.P., Fernandes F., Moreira L.M.

Reference: Applied and Environmental Microbiology (2013) 79(9): 3009-3020

Extracellular DNA shields against aminoglycosides in Pseudomonas aeruginosa biofilms

by Chiang W.C., Nilsson M., Jensen P.O., Hoiby N., Nielsen T.E., Givskov M., Tolker-Nielsen T.

Reference: Antimicrobial Agents and Chemotherapy (2013) 57(5): 2352-2361

Comparative genomic analysis of rapid evolution of an extreme-drug-resistant (EDR) Acinetobacter baumannii clone

by Tan S.Y., Chua S. L., Liu Y., Høiby N., Andersen L.P., Givskov M., Song Z., Yang L.

Reference: Genome Biology and Evolution (2013) 5(5): 807-818

Research Cluster(s): Biofilm Biology

Synthesis and biological evaluation of triazole-containing N-acyl homoserine lactones as quorum sensing modulators

by Stacy D.M., Le Quement S.T., Hansen C.L., Clausen J.W., Tolker-Nielsen T., Brummond J.W., Givskov M., Nielsen T.E., Blackwell H.E.

Reference: Organic and Biomolecular Chemistry (2013) 11(6): 938-954

Identification of Burkholderia cenocepacia strain H111 virulence factors using nonmammalian infection hosts

by Schwager S., Agnoli K., Köthe M., Feldmann F., Givskov M., Carlier A., Eberl L.

Reference: Infection and Immunity (2013) 81(1): 143-153

Kinetic model for signal binding to the quorum sensing regulator LasR

by Claussen A., Jakobsen T.H., Bjarnsholt T., Givskov M., Welch M., Ferkinghoff-Borg J., Sams T.

Reference: International Journal of Molecular Sciences (2013) 17(7): 13360-13376

Programme(s): Biofilm Detection and Control

Biofilm formation by Staphylococcus epidermidis on peritoneal dialysis catheters and the effects of extracellular products from Pseudomonas aeruginosa

by Pihl M., Arvidsson A., Skepö M., Nilsson M., Givskov M., Tolker-Nielsen T., Svensäter G., Davies J.R.

Reference: Pathogens and Disease (2013) 67(3): 192-198

Identification of LasR ligands through a virtual screening approach

by Skovstrup S., Le Quement S.T., Hansen T., Jakobsen T.H., Harmsen M., Tolker-Nielsen T., Nielsen T.E., Givskov M., Taboureau O.

Reference: ChemMedChem (2013) 8(1): 157-163

Complete genome sequence of the cystic fibrosis pathogen Achromobacter xylosoxidans NH44784-1996 complies with important pathogenic phenotypes

by Jakobsen T.H., Hansen M.A., Jensen P.Ø., Hansen L., Riber L., Cockburn A., Kolpen M., Rønne Hansen C., Ridderberg W., Eickhardt S., Hansen M., Kerpedjiev P., Alhede M., Qvortrup K., Burmølle M., Moser C., Kühl M., Ciofu O., Givskov M., Sørensen S.J., Høiby N., Bjarnsholt T.

Reference: PLoS ONE (2013) 8(7): e68484

The exopolysaccharide gene cluster Bcam1330-Bcam1341 is involved in Burkholderia cenocepacia biofilm formation, and its expression is regulated by c-di-GMP and Bcam1349

by Fazli M., McCarthy Y., Givskov M., Ryan R.P., Tolker-Nielsen T.

Reference: MicrobiologyOpen (2013) 2(1): 105-122

Expression of Fap amyloids in Pseudomonas aeruginosa, P. fluorescens, and P. putida results in aggregation and increased biofilm formation

by Dueholm M.S., Søndergaard M.T., Nilsson M., Christiansen G., Stensballe A., Overgaard M.T., Givskov M., Tolker-Nielsen T., Otzen D.E., Nielsen P. H.

Reference: MicrobiologyOpen (2013) 2(3): 365-382

Targeting quorum sensing in Pseudomonas aeruginosa biofilms: current and emerging inhibitors

by Jakobsen T.H., Bjarnsholt T., Jensen P.Ø., Givskov M., Høiby N.

Reference: Future Microbiology (2013) 8(7): 901-921

Research Cluster(s): Biofilm Biology

First case of E. anophelis outbreak in an intensive-care unit

by Teo J., Tan S.Y., Tay M., Ding Y., Kjelleberg S., Givskov M., Lin R.T.P., Yang L.

Reference: The Lancet (2013) 382(9895): 855-856

Research Cluster(s): Biofilm Biology

The catabolite repression control protein Crc plays a role in the development of antimicrobial-tolerant subpopulations in Pseudomonas aeruginosa biofilms

by Zhang L., Chiang W.-C., Gao Q., Givskov M., Tolker-Nielsen T., Yang L., Zhang G.

Reference: Microbiology (2012) 158(12): 3014-3019

Research Cluster(s): Biofilm Biology

Beyond nutrition: Health-promoting foods by quorum-sensing inhibition

by Givskov M.

Reference: Future Microbiology (2012) 7(9): 1025-1028

Research Cluster(s): Biofilm Biology

Bursting the bubble on bacterial biofilms: A flow cell methodology

by Crusz S.A., Popat R., Rybtke M.T., Cámara M., Givskov M., Tolker-Nielsen T., Diggle S.P., Williams P.

Reference: Biofouling (2012) 28(8): 835-842

Fluorescence-based reporter for gauging cyclic di-GMP levels in Pseudomonas aeruginosa

by Rybtke M.T., Borlee B.R., Murakami K., Irie Y., Hentzer M., Nielsen T.E., Givskov M., Parsek M.R., Tolker-Nielsen T.

Reference: Applied and Environmental Microbiology (2012) 78(15): 5060-5069

Programme(s): Biofilm Detection and Control

Interactions between polymorphonuclear leukocytes and P. aeruginosa biofilms on silicone implants in vivo

by van Gennip M., Christensen L.D., Alhede M., Qvortrup K., Jensen P. Ø., Høiby N., Givskov M., Bjarnsholt T.

Reference: Infection and Immunity (2012) 80: 2601-2607

Programme(s): Biofilm Detection and Control

Synergistic antibacterial efficacy of early combination treatment with tobramycin and quorum-sensing inhibitors against Pseudomonas aeruginosa in an intraperitoneal foreign-body infection mouse model

by Christensen L.D., van Gennip M., Jakobsen T.H., Alhede M., Hougen H.P., Høiby N., Bjarnsholt T., Givskov M.

Reference: Journal of Antimicrobial Chemotherapy (2012) 67(5): 1198-1206

Programme(s): Biofilm Detection and Control

Ajoene, a sulfur-rich molecule from garlic, inhibits genes controlled by quorum sensing

by Jakobsen T.H., van Gennip M., Phipps R.K., Shanmugham S., Christensen L.D., Alhede M., Skindersoe M.E., Rasmussen T.B., Friedrich K., Uthe F., Jensen P.Ø., Moser C., Nielsen K.F., Eberl L., Larsen T.O., Tanner D., Høiby N., Bjarnsholt T., Givskov M.

Reference: Antimicrobial Agents and Chemotherapy (2012) 56(5): 2314-2325

Programme(s): Biofilm Detection and Control

Food as a source for quorum sensing inhibitors: Iberin from horseradish revealed as a quorum sensing inhibitor of Pseudomonas aeruginosa

by Jakobsen T.H., Bragason S.K., Phipps R.K., Christensen L.D., van Gennip M., Alhede M., Skindersoe M., Larsen T.O., Høiby N., Bjarnsholt T., Givskov M.

Reference: Microbiology (2012) 78(7): 2410-2421

Programme(s): Biofilm Detection and Control

Petasis three-component coupling reactions of hydrazides for the synthesis of oxadiazolones and oxazolidinones

by Le Quement S.T., Flagstad T., Mikkelsen R.J.T., Hansen M.R., Givskov M., Nielsen T.E.

Reference: Organic Letters (2012) 14(2): 640-643

Combination of microscopic techniques reveals a comprehensive visual impression of biofilm structure and composition

by Alhede M., Qvortrup K., Liebrechts R., Høiby N., Givskov M., Bjarnsholt T.

Reference: FEMS Immunology and Medical Microbiology (2012) 65(2): 335-342

Solid-phase synthesis of structurally diverse heterocycles by an amide–ketone condensation/N-acyliminium Pictet–Spengler sequence

by Komantnyy V.V., Givskov M., Nielsen T.E.

Reference: Chemistry – A European Journal (2012) 18(52): 16793-16800