News & Events
The role of phosphatidylglycerol in virulence factor assembly and cationic antimicrobial peptide resistance
Speaker(s): Chen Qingyan, PhD Student, SCELSE
When: 15 March 2017 (3:00 - 4:00pm)
Where: SBS CR3 (Level 1)
Type: Seminars

Abstract

Enterococcus faecalis is a Gram-positive bacterium which can become highly resistant to various antibiotics. The Enterococci pathogen primarily rely on a conserved secretory (Sec) and sorting pathway for virulence factor assembly. The anionic lipid phosphatidylglycerol (PG) is a major lipid in bacterial membranes and reducing the PG composition in the cell membrane of Bacillus subtilis results in the delocalisation of SecA, a highly conserved cell membrane associated subunit in the Sec pathway. Hence, we aim to investigate the significance of PG in the localisation of virulence factor assembly machinery. Furthermore, we also seek to examine interactions between the anionic PG and cationic antimicrobial peptides (CAMPs), which are known to target anionic domains. Together, these studies will shed light on the mechanisms of virulence factor assembly in E. faecalis.