Publications
Engineering PQS biosynthesis pathway for enhancement of bioelectricity production in Pseudomonas aeruginosa microbial fuel cells
Reference: PLoS ONE (2013) 8(5): e63129

The biosynthesis of the redox shuttle, phenazines, in Pseudomonas aeruginosa, an ubiquitous microorganism in wastewater microflora, is regulated by the 2-heptyl-3,4-dihydroxyquinoline (PQS) quorum-sensing system. However, PQS inhibits anaerobic growth of P. aeruginosa. We constructed a P. aeruginosa strain that produces higher concentrations of phenazines under anaerobic conditions by over-expressing the PqsE effector in a PQS negative δpqsC mutant. The engineered strain exhibited an improved electrical performance in microbial fuel cells (MFCs) and potentiostat-controlled electrochemical cells with an approximate five-fold increase of maximum current density relative to the parent strain. Electrochemical analysis showed that the current increase correlates with an over-synthesis of phenazines. These results therefore demonstrate that targeting microbial cell-to-cell communication by genetic engineering is a suitable technique to improve power output of bioelectrochemical systems.

http://search.proquest.com/docview/1353319396?accountid=12763

Wang V. B., Chua S.-L., Cao B., Seviour T., Nesatyy V. J., Marsili E., Kjelleberg S., Givskov M., Tolker-Nielsen T., Song H., Loo J. S. C. and Yang L.

Published By
Wang V. B., Chua S. L., Cao B., Seviour T., Nesatyy V.J., Marsili E., Kjelleberg S., Givskov M., Tolker-Nielsen T., Song H., Loo J. S. C., Yang L.